Figure 4

miR-214-5p expression in liver cells, including stellate cells. (A) miR-214-5p expression in mouse stellate cells during primary culture was analyzed using real-time PCR. Stellate cells were isolated from mouse livers and cultured for 1, 4 or 7 days. The results are expressed relative to the expression of miR-214-5p at day 1. *P < 0.05. (B) The expression of α- smooth muscle actin (α-SMA), the type 1 collagen alpha 1 chain (Col1a1), platelet-derived growth factor receptor (PDGFR)-β, fibronectin (FN)1 and transforming growth factor (TGF)-β1 mRNA in primary-cultured mouse stellate cells was analyzed using real-time PCR. The results are expressed relative to the expression of the same mRNA at day 1. **P < 0.01. (C) miR-214-5p expression in HepG2, Huh7 and LX-2 cells was analyzed using real-time PCR. The results are expressed relative to the expression of miR-214-5p in HepG2. **P < 0.01. (D) miR-214-5p expression in hepatocytes (Hc), the non-parenchymal cell (NPC) fraction, and the hepatic stellate cell (HSC) fraction. The cells were isolated from intact mouse livers. The miR-214-5p expression in Hc, the NPC fraction, and the HSC fraction was analyzed using real-time PCR. The results are expressed relative to the expression of miR-214-5p in hepatocytes. **P < 0.01.