Figure 2

miR-214-5p expression in mouse livers with fibrosis induced by an methionine- and choline-deficient diet. (A) Sirius red staining (left and middle panels) and α-smooth muscle actin (SMA) immunostaining (right panels) of mouse liver tissues. Collagen deposition and an increase in α-SMA-positive cells were evident around the central vein area of the liver of mice that received the MCDD for 15 weeks. Scale bars, 100 μm (left and middle panels) and 200 μm (right panels). P, portal vein. C, central vein. (B) The mRNA expression of α-smooth muscle actin (α-SMA), the type 1 collagen alpha 1 chain (Col1a1), platelet-derived growth factor receptor (PDGFR)-β, transforming growth factor (TGF)-β1, fibronectin (FN)1, discoidin domain receptor (DDR)2, and β1 integrin (ITGB1) in fibrotic mouse livers was analyzed using real-time PCR. The results are expressed relative to mRNA expression at 5 weeks of the methionine- and choline-control diet (MCCD). *P < 0.05. **P < 0.01. (C) miR-214-5p expression in fibrotic mouse livers was analyzed using real-time PCR. The results are expressed relative to the expression of miR-214-5p at 5 weeks of the MCCD. *P < 0.05. **P < 0.01.