| |
K
d
for binding to CI (in nM) in the presence of 10-fold molar excess
|
|---|
| |
-
|
(GPP)10
|
(POG)5
|
(GPO)10
|
|---|
|
ProMMP-2
|
7.1 ± 0.1
|
42.1 ± 39.1
|
155.5 ± 40.3
|
155.5 ± 50.2
|
|
ActMMP-2
|
250.0 ± 30.0
|
n.d.
|
n.d.
|
2,710.0 ± 90.0
|
- aPro- or actMMP-2 (100 nM) was passed over collagen type I (CI) immobilized to a sensor chip in the presence or absence of 10-fold molar excesses of (GPP)10, (POG)5, or (GPO)10. Reagents were dissolved in matrix metalloproteinase (MMP) activity buffer, and MMP-2 activity was specifically blocked by 1 μM Ro 28-2653. K
d
values of MMP-2 binding to CI were determined by surface plasmon resonance (SPR) analysis and are shown as mean values ± SD from at least three experiments. Hyp, hydroxyproline; GPP, Gly-Pro-Pro; GPO, Gly-Pro-Hyp triplets; n.d., not determined.
