Fig. 4

Functional comparison of human aHSCs and rHSCs. a EdU staining images showing the influence of RM on basal and PDGF-BB-induced HSC proliferation. b The percentage proliferative cells, calculated as the ratio of EdU-positive nuclei over the total (DAPI) nuclei. c The PDGF-BB (20 ng/mL) induced fold change in proliferation for aHSCs and rHSCs, relative to their basal condition. d Fluorescent images depicting the basal and TGFβ-induced (10 ng/mL) proteolytic digestion (green) of the fluorescein-labeled gelatin substrate by aHSCs and rHSCs. e The quantified basal and TGFβ-induced matrix metalloproteinase activity of aHSCs and rHSCs presented as the percentage green stained area over the total image area. f The PDGF-BB-inducible migration of aHSCs and rHSCs was assessed in a transwell migration assay. The aHSCs and rHSCs were seeded in collagen-coated Boyden chambers and stimulated with PDGF-BB (20 ng/mL) or with its solvent as a control, in the lower compartment. Results are presented as the number of migrated cells in each condition. The presented results are from three different donors. ns not significant, p ≥ 0.05, *p < 0.05