Figure 3

BGM release from ex vivo cultures of bovine explants (BEX), measured at day 3 and 17 of culturing on five replicates. w/o = cultures without any stimulation; MI = metabolically inactive cultures by the treatment with liquid nitrogen; T+O = catabolic cytokine stimulation with TNF-α (20 ng/mL) and oncostatin (10 ng/mL); T+O+GM6001 = catabolic cytokines TNF-α (20 ng/mL) and oncostatin (10 ng/mL) supplemented with selective MMP inhibitor GM6001; T+O+E64 = catabolic cytokines TNF-α (20 ng/mL) and oncostatin (10 ng/mL) supplemented with selective cysteine protease inhibitor E64. (*** P < 0.001).