Figure 2
A. Effect of combined pentoxifylline-trolox on mRNA expression of profibrotic genes in a kinetic manner: Twenty four hours kinetics of mRNA expression of TGF-β1, PAI-1, Col Iα1, and RhoB. Pentoxifylline and trolox synergize to inhibit TGFβ1 at early point time, subsequent inhibition of TGFβ1 targets such as PAI-1 and Col Iα1. Treatment doesn't affect RhoB mRNA expression. P: Pentoxifylline treatment, T: Trolox treatment, PT: Pentoxifylline-trolox treatment. 10 refers to treatment dose in μg/ml. *: p < 0.05, **: p < 0.01, ***: p < 0.005 according to kruskal-Wallis test. B. Protein expression of PAI-1 in control and pentoxifylline-trolox treated cells: Combined pentoxifylline-trolox treatment for 24 hours inhibits PAI-1 protein expression more effectively than pentoxifylline alone or trolox alone; GAPDH is used as a housekeeping gene. C: control, P: Pentoxifylline treatment, T: Trolox treatment, PT: Pentoxifylline-trolox treatment. 10 refers to treatment dose in μg/ml.