Fig. 3
From: Activation of hepatic stellate cell in Pten null liver injury model
Pten null livers develop progressive fibrosis that is attenuated by AKT2 loss. a Periductal (arrowhead) and pericellular (arrows) staining of Sirius Red are obvious in the 6- and 9-month-old Pten null livers, indicating fibrosis development. In older mice (12 months of age), more intensive Sirius Red staining is observed. In Pten and Akt2 double mutants (Pten/Akt2 null), minimum staining for Sirius Red is observed (bottom row). Top row, controls; middle two rows, Pten null livers. b Sirius Red staining in tumor samples from the Pten null mice. c Sirius Red stained areas were quantified vs. non-stained areas. Percentage of total area that is positive for Sirius Red is reported. Each circle represents an animal. Open circle, control; solid red circle, Pten null