Fig. 5
From: Protective role for miR-9-5p in the fibrogenic transformation of human dermal fibroblasts
miR-9-5p prevents bleomycin-induced skin fibrosis. a, b miR-9-5p expression was assayed by qRT-PCR in the skin (a) and plasma (b) after daily subcutaneous injections of 10 μg bleomycin for 14 and 28 days. c H&E staining and Masson’s trichrome blue staining for collagen in skin samples from mice administered 1 × 108 ifu of lenti-SC (control) or lenti-miR-9 for 4 days followed by daily subcutaneous injections of 10 μg bleomycin for 4 weeks. Scale bars: 100 μm. d Quantitative evaluation of dermal thickness from the same samples as in (c), calculated as the ratio between dermis and total skin thickness. e, f qRT-PCR analysis for mRNA expression of Col1α1 (e) and FN (f) in skin samples from mice administered 1 × 108 ifu of lenti-SC (control) or lenti-miR-9 for 4 days followed by daily subcutaneous injections of 10 μg bleomycin for 4 weeks. All bar graphs represent mean ± SEM (n = 6 mice in each group), *P < 0.05 and ***P < 0.001 compared to mice given control lentivirus and saline-treated and # P < 0.05 compared to mice given control lentivirus and bleomycin-treated