Fig. 1
From: Protective role for miR-9-5p in the fibrogenic transformation of human dermal fibroblasts
miR-9-5p is induced by TGF-β1 and reduces TGFBR2 expression. a miR-9-5p expression was assayed by qRT-PCR in HDFs stimulated with TGF-β1 (5 ng/ml) at the indicated times. b qRT-PCR analysis for mRNA expression of TGFBR2 in HDFs transfected with 40-nM pre-miR-9-5p or pre-miR-NC. c Western blot analysis (left) and quantification (right) of TGFBR2 expression in HDFs transfected with 40-nM pre-miR-9-5p or pre-miR-NC (a.u., arbitrary units). The bar graph shows values after correction by GAPDH expression and normalized to control conditions. The bar graphs show mean ± SEM of three independent experiments, *P < 0.05 and ***P < 0.001 compared to control cells