Fig. 4

Active Mtb infection and disease activates TGFβ and suppresses SMAD-2/3 signaling. a–f Quantification of IHC. Median pixel intensity (MPI) was calculated from red, green, and blue color channels of each micrograph and plotted by stage of drug therapy. Bars in each column represent medians. Kruskal–Wallis test had p values of less than 0.0001 for TGFβ and αSMA, pSMAD-2/3 (0.0006), L-TGFβ (0.0148), and collagen I and CD163 (not significant). *p < 0.05. **p < 0.01. ***p < 0.001. ****p < 0.0001