Fig. 5

Hepatocyte proliferation and control of the cell cycle. Representative images of liver sections from aged-matched wild-type (WT) and Glmp ^gt/gt mice (a) labeled for active Ki67 and counterstained with Mayer’s hematoxylin indicate cell proliferation (arrows). Scale bar 100 μm. b Quantification of proliferating hepatocytes and c number of hepatocyte nuclei in aged-matched WT and Glmp ^gt/gt liver sections (n = 4, representing six random images and at least 1000 hepatocytes from four individuals/genotype/age). Relative mRNA expression at designated age-points of the proliferation marker d Ccnd1 and one of its inhibitor e Cdkn2a, and the expression of senescence marker f Cyr61 at 3.5 months were analyzed using qPCR in mouse livers from WT and Glmp ^gt/gt mice (n = 4, *p < 0.05, **p < 0.01, ***p < 0.005 vs. WT). Values are presented as mean ± s.e.m