Fig. 3

Determination of liver collagen expression and cross-linking. Representative images of paraffin-embedded liver sections from aged-matched wild-type (WT) and Glmp ^gt/gt mice, stained with acid fuchsin orange G (blue) (a) revealed excess depositions of collagen in Glmp ^gt/gt liver parenchyma from 1 month of age (arrows). Scale bar 100 μm. b Quantification of collagen in age-matched WT and Glmp ^gt/gt livers were determined by analyzing liver hydroxyproline content (n = 3–4, *p < 0.05, **p < 0.01, ***p < 0.005 vs. WT). Values are presented as mean ± s.e.m