Fig. 8
From: Differential effects of hyaluronan synthase 3 deficiency after acute vs chronic liver injury in mice
Hepatic Mmp and Timp content in the livers from wild-type and Has3−/− mice. The mice were exposed to CCl4 for 5 weeks, twice per week, then were euthanized 72 h after the final CCl4 exposure. Control animals received olive oil (oil) injections. Hepatic a Mmp2, b Mmp9, c Timp1 and d Mmp13 transcript accumulation was evaluated using real-time PCR. Data are expressed as fold change over each strain’s baseline (oil) after normalization to 18S. e Representative immunoblot of hepatic pro and active MMP13 protein. GAPDH is used to demonstrate equal loading. f Semi-quantification MMP13 band densities expressed as relative amount of active MMP13 relative to pro MMP13. N = 5–6 mice per experimental group. *P < 0.05 compared between genotypes