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Fig. 7 | Fibrogenesis & Tissue Repair

Fig. 7

From: Differential effects of hyaluronan synthase 3 deficiency after acute vs chronic liver injury in mice

Fig. 7

Liver fibrosis in wild-type and Has3−/− mice after chronic CCl4 exposure. The mice were exposed to CCl4 for 5 weeks, twice per week, and were euthanized 72 h after the final CCl4 exposure. Control animals received olive oil (oil) injections. a Sirius red was used to localize extracellular matrix accumulation in the livers from the control and CCl4-treated mice (red bands around vascular units and extending from and connecting neighboring central veins, ×100 magnification). b Semi-quantification of Sirius red staining expressed as a percent of total tissue area. Similar quantification of Sirius red staining intensity was also performed, and no differences were observed between groups (data not shown). c Hepatic hydroxyproline concentration was determined using a biochemical assay from which approximate collagen content was calculated (see the “Methods” for further details). N = 5–6 mice per experimental group

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