Fig. 4

Hepatic and plasma chemokine content in wild-type and Has3−/− mice. Forty-eight hours after CCl₄ exposure, the mice were euthanized and the livers were collected to determine the relative amounts of a Ccl2, b Cxcl1, and c Cxcl10 transcripts in both genotypes. Here and throughout the manuscript, data were calculated using the 2^−ΔΔCt method after normalization to 18S and are expressed as fold change over each genotype’s own baseline (oil). Plasma isolated from blood collected at the same time points was used to determine the relative amounts of CCL2 (d), CXCL1 (e), and CXCL10 (f) peptides using a protein array. For this assay, plasma samples were pooled per experimental group (n = 6–8 mice). The bar graphs in d–f were created after semi-quantification of chemokine spot density from the array after densitometric analysis (spots not shown). *P < 0.05 compared between genotypes at the same time point