Fig. 2

Liver injury and steatosis in wild-type and Has3−/− mice. The mice were exposed to CCl₄ and euthanized 24, 48, 72, or 96 h later. Control animals received an olive oil (oil) injection. a Plasma ALT activity was determined using an enzymatic assay. b Histopathologic assessment of the percent necrosis at baseline and 48 h after CCl₄ exposure. c Total hepatic triglycerides were measured using a biochemical assay. d Representative histology in the wild-type and Has3−/− mice (200×). Asterisks denote central veins, and plus signs denote the portal vein of the portal triad. The necrotic areas are outlined with yellow dashed line in the 48 and 72 h images, and the area of macrovesicular steatosis is identified by black arrows in the 24 h images. In every figure of this manuscript, white bars indicate wild-type mice while black bars indicate Has3−/− mice. *P < 0.05 compared between genotypes at the same time point. N = 4–7 mice per experimental group