Table 1 Verification of gene expression changes by RT-qPCR
Gene symbol | Gene name | Primer sequences | Expression of fold change (log2)* | Gene function | |
|---|---|---|---|---|---|
Gene Chip | q-PCR | ||||
SP1 | Specific protein 1 | F:5′-ACTGAGATCCCCAAAACACC-3′; R:5′-TTCTCTGCCCTCACTCTTGA-3′ | 2.00 | 3.81 | Transcriptional factor |
STAT3 | Signal transducer and activator of transcription 3 | F:5′-TCACTTGGGTGGAAAAGGAC-3′; R:GGAATGTCGGGGTAGAGGTAG-3′ | 1.89 | 2.35 | Transcriptional factor |
Jun | Jun proto-oncogene | F:5′-CCTTCTACGACGATGCCCTC-3′; R:5′-GGTTCAAGGTCATGCTCTGTTT-3′ | 3.01 | 3.94 | Transcriptional factor |
Fas | Fas cell surface death receptor | F:5′-GCAGACATGCTGTGGATCTGG-3′; R:5′-TCACAGCCAGGAGAATCGCAG-3′ | 5.10 | 2.18 | Transcriptional factor |
FGF7 | Fibroblast growth factor 7 (keratinocyte growth factor) | F:5′-ACGGCTACGAGTGTGAACTGT-3′; R:5′-TTTCACTTTGCCTCGTTTGTC-3′ | 75.96 | 42 | Growth factor |
BDNF | Brain-derived neurotrophic factor | F:5′-GCCTCCTCTACTCTTTCTGCTG-3′; R:5′-TGTGACCCACTCGCTAATACTG-3′ | 2.68 | 5.76 | Growth factor |
VEGFD (FIGF) | c-fos induced growth factor (vascular endothelial growth factor D) | F:5′-AGCACCTCCTACATCTCCAAAC-3′; R:5′-CATTCATCTTCTTCTGGGGTCT-3′ | 4.82 | 5.76 | Growth factor |
IGF1 | Insulin-like growth factor 1 | F:5′-AAAATCAGCAGCCTTCCAACT-3′; R:5′-CCTGTGGGCTTGTTGAAGTAA-3′ | 2.17 | 3.13 | Growth factor |
PTN | Pleiotrophin | F:5′-CTCTGCACAATGCTGACTGTC-3′; R:5′-CTTTGACTCCGCTTGAGGCTT-3′ | 31.78 | 36 | Growth factor |
PDGFα | Platelet-derived growth factor, alpha | F:5′-TGGCTCGAAGTCAGATCCACA-3′; R:5′-TTCTCGGGCACATGGTTAATG-3′ | 1.61 | 1.229 | Growth factor |
IGFBP3 | Insulin-like growth factor binding protein 3 | F:5′-TCCAGGAAACATCAGTGAGTCCGA-3′; R:5′-CATACTTGTCCACACACCAGCAGA-3′ | 22.75 | 27.84 | Insulin-like growth factor binding protein |
Col I | collagen, type I | F:5′-GCTCCTCTTAGGGGCCACT-3′; R:5′-CCACGTCTCACCATTGGGG-3′ | 3.56 | 1.6 | Fibrogenesis |
FN1 | Fibronectin 1 | F:5′-TTCAAGTGTGATCCCCATGAAG-3′; R:5′-CAGGTCTACGGCAGTTGTCA-3′ | 7.34 | 5.26 | Fibrogenesis |
TIMP2 | Tissue inhibitors of metalloproteinase 2 | F:5′-CTGGACGTTGGAGGAAAGAAG-3′; R:5′-CTGGGTGATGCTAAGCGTGTC-3′ | 2.01 | 3.456 | Matrix remodeling |
TIMP3 | Tissue inhibitors of metalloproteinase 3 | F:5′-GCAAGGGCCTCAATTACCG-3′; R: 5′-AGGCGTAGTGTTTGGACTGATA-3′ | 8.57 | 10.389 | Matrix remodeling |
MMP2 | Matrix metalloproteinase 2 | F:5′-GTGTCTTCCCCTTCACTTTCCT-3′; R:5′-CATCATCGTAGTTGGTTGTGGT-3′ | 8.07 | 10.24 | Matrix remodeling |
IL6 | Interleukin 6 | F:5′-GGAGAGGAGACTTCACAGAGGA-3′; R:5′-ATTTCCACGATTTCCCAGAGA-3′ | 3.00 | 1.74 | Inflammatory factor |
CXCR7 | Chemokine (C-X-C motif) receptor 7 | F:5′-AGCCTGGCAACTACTCTGACA-3′; R:5′-GAAGCACGTTCTTGTTAGGCA-3′ | 11.31 | 14.42 | Chemokine receptor |
CXCL11 | Chemokine (C-X-C motif) ligand 11 | F:5′-GGCTTCCTTATGTTCAAACAGGG-3′; R:5′-GCCGTTACTCGGGTAAATTACA-3′ | 2.03 | 0.65 | Chemokine |
CXCL12 | Chemokine (C-X-C motif) ligand 12 | F:5′-ACTGTGCCCTTCAGATTGTTG-3′; R:5′-CAGCCTTTCTCTTCTTCTGTCG-3′ | 44.84 | 34.95 | Chemokine |
CASP2 | Caspase 2 | F:5′-GCAAGATGGAAAGAACCACAC-3′; R:5′-GCAAGATGGAAAGAACCACAC-3′ | 1.83 | 1.7 | Apoptin |
DLK1 | Delta-like 1 homolog (Drosophila) | F:5′-AGTGCGAAACCTGGGTGTC-3′; R:5′-GCCTCCTTGTTGAAAGTGGTCA-3′ | 18.95 | 19.784 | Tumor repressor, cell differentiation |
AKT3 | Thymoma viral proto-oncogene homolog 3 | F:5′-GTGGACTTACCTTATCCCCTCA-3′; R:5′-TTGGCTTTGGTCGTTCTGTTT-3′ | 2.50 | 3.27 | Serine/threonine protein kinase |
GNG2 | Guanine nucleotide binding protein (G protein), gamma 2 | F:5′-GAAGCCAACATCGACAGGAT-3′; R:5′-GTTTTCTGAGGCTGGGACTG-3′ | 5.71 | 5.5 | Signaling transduction |
MDM2 | E3 ubiquitin protein ligase | F:5′-AGATCCTGAGATTTCCTTAGCTGACT-3′; R:5′-TCTCACGAAGGGTCCAGCATCT-3′ | 3.77 | 4 | Ubiquitination |
Wnt5a | Wingless-related MMTV integration site 5A | F:5′-CAACTGGCAGGACTTTCTCAA-3′; R:5′-CATCTCCGATGCCGGAACT-3′ | 2.35 | 3.73 | Modulator of Wnt signaling |