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Table 1 Verification of gene expression changes by RT-qPCR

From: Transcriptomic analysis of the effects of Toll-like receptor 4 and its ligands on the gene expression network of hepatic stellate cells

Gene symbol Gene name Primer sequences Expression of fold change (log2)* Gene function
Gene Chip q-PCR
SP1 Specific protein 1 F:5′-ACTGAGATCCCCAAAACACC-3′; R:5′-TTCTCTGCCCTCACTCTTGA-3′ 2.00 3.81 Transcriptional factor
STAT3 Signal transducer and activator of transcription 3 F:5′-TCACTTGGGTGGAAAAGGAC-3′; R:GGAATGTCGGGGTAGAGGTAG-3′ 1.89 2.35 Transcriptional factor
Jun Jun proto-oncogene F:5′-CCTTCTACGACGATGCCCTC-3′; R:5′-GGTTCAAGGTCATGCTCTGTTT-3′ 3.01 3.94 Transcriptional factor
Fas Fas cell surface death receptor F:5′-GCAGACATGCTGTGGATCTGG-3′; R:5′-TCACAGCCAGGAGAATCGCAG-3′ 5.10 2.18 Transcriptional factor
FGF7 Fibroblast growth factor 7 (keratinocyte growth factor) F:5′-ACGGCTACGAGTGTGAACTGT-3′; R:5′-TTTCACTTTGCCTCGTTTGTC-3′ 75.96 42 Growth factor
BDNF Brain-derived neurotrophic factor F:5′-GCCTCCTCTACTCTTTCTGCTG-3′; R:5′-TGTGACCCACTCGCTAATACTG-3′ 2.68 5.76 Growth factor
VEGFD (FIGF) c-fos induced growth factor (vascular endothelial growth factor D) F:5′-AGCACCTCCTACATCTCCAAAC-3′; R:5′-CATTCATCTTCTTCTGGGGTCT-3′ 4.82 5.76 Growth factor
IGF1 Insulin-like growth factor 1 F:5′-AAAATCAGCAGCCTTCCAACT-3′; R:5′-CCTGTGGGCTTGTTGAAGTAA-3′ 2.17 3.13 Growth factor
PTN Pleiotrophin F:5′-CTCTGCACAATGCTGACTGTC-3′; R:5′-CTTTGACTCCGCTTGAGGCTT-3′ 31.78 36 Growth factor
PDGFα Platelet-derived growth factor, alpha F:5′-TGGCTCGAAGTCAGATCCACA-3′; R:5′-TTCTCGGGCACATGGTTAATG-3′ 1.61 1.229 Growth factor
IGFBP3 Insulin-like growth factor binding protein 3 F:5′-TCCAGGAAACATCAGTGAGTCCGA-3′; R:5′-CATACTTGTCCACACACCAGCAGA-3′ 22.75 27.84 Insulin-like growth factor binding protein
Col I collagen, type I F:5′-GCTCCTCTTAGGGGCCACT-3′; R:5′-CCACGTCTCACCATTGGGG-3′ 3.56 1.6 Fibrogenesis
FN1 Fibronectin 1 F:5′-TTCAAGTGTGATCCCCATGAAG-3′; R:5′-CAGGTCTACGGCAGTTGTCA-3′ 7.34 5.26 Fibrogenesis
TIMP2 Tissue inhibitors of metalloproteinase 2 F:5′-CTGGACGTTGGAGGAAAGAAG-3′; R:5′-CTGGGTGATGCTAAGCGTGTC-3′ 2.01 3.456 Matrix remodeling
TIMP3 Tissue inhibitors of metalloproteinase 3 F:5′-GCAAGGGCCTCAATTACCG-3′; R: 5′-AGGCGTAGTGTTTGGACTGATA-3′ 8.57 10.389 Matrix remodeling
MMP2 Matrix metalloproteinase 2 F:5′-GTGTCTTCCCCTTCACTTTCCT-3′; R:5′-CATCATCGTAGTTGGTTGTGGT-3′ 8.07 10.24 Matrix remodeling
IL6 Interleukin 6 F:5′-GGAGAGGAGACTTCACAGAGGA-3′; R:5′-ATTTCCACGATTTCCCAGAGA-3′ 3.00 1.74 Inflammatory factor
CXCR7 Chemokine (C-X-C motif) receptor 7 F:5′-AGCCTGGCAACTACTCTGACA-3′; R:5′-GAAGCACGTTCTTGTTAGGCA-3′ 11.31 14.42 Chemokine receptor
CXCL11 Chemokine (C-X-C motif) ligand 11 F:5′-GGCTTCCTTATGTTCAAACAGGG-3′; R:5′-GCCGTTACTCGGGTAAATTACA-3′ 2.03 0.65 Chemokine
CXCL12 Chemokine (C-X-C motif) ligand 12 F:5′-ACTGTGCCCTTCAGATTGTTG-3′; R:5′-CAGCCTTTCTCTTCTTCTGTCG-3′ 44.84 34.95 Chemokine
CASP2 Caspase 2 F:5′-GCAAGATGGAAAGAACCACAC-3′; R:5′-GCAAGATGGAAAGAACCACAC-3′ 1.83 1.7 Apoptin
DLK1 Delta-like 1 homolog (Drosophila) F:5′-AGTGCGAAACCTGGGTGTC-3′; R:5′-GCCTCCTTGTTGAAAGTGGTCA-3′ 18.95 19.784 Tumor repressor, cell differentiation
AKT3 Thymoma viral proto-oncogene homolog 3 F:5′-GTGGACTTACCTTATCCCCTCA-3′; R:5′-TTGGCTTTGGTCGTTCTGTTT-3′ 2.50 3.27 Serine/threonine protein kinase
GNG2 Guanine nucleotide binding protein (G protein), gamma 2 F:5′-GAAGCCAACATCGACAGGAT-3′; R:5′-GTTTTCTGAGGCTGGGACTG-3′ 5.71 5.5 Signaling transduction
MDM2 E3 ubiquitin protein ligase F:5′-AGATCCTGAGATTTCCTTAGCTGACT-3′; R:5′-TCTCACGAAGGGTCCAGCATCT-3′ 3.77 4 Ubiquitination
Wnt5a Wingless-related MMTV integration site 5A F:5′-CAACTGGCAGGACTTTCTCAA-3′; R:5′-CATCTCCGATGCCGGAACT-3′ 2.35 3.73 Modulator of Wnt signaling
  1. *P < 0.05, gene mRNA expression in JS1 cells when compared to JS2