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Fig. 3 | Fibrogenesis & Tissue Repair

Fig. 3

From: Thromboxane promotes smooth muscle phenotype commitment but not remodeling of hypoxic neonatal pulmonary artery

Fig. 3

Third-generation pulmonary artery strips from newborn (control D0), normoxic 3-day-old (control D3), and hypoxic 3-day-old piglets (PPHN D3) cultured for 72 h in aerated media with or without 10 % fetal bovine serum (favoring expansion of synthetic or contractile phenotype, respectively). Tissue culture in Krebs physiological buffer also studied as a baseline control. Tissue strips were randomized to daily addition of thromboxane mimetic U46619 for 72 h. Tissue DNA synthesis was evaluated by 3H-thymidine incorporation and protein synthesis by incorporation of 3H-leucine. Labeled amino acid or nucleotide was added for the final 24 h; tissue uptake expressed is as dpm/mg protein. Protein contents for tissue homogenates from all groups were obtained by Bradford method. a [i] DNA synthesis, [ii] active protein synthesis in PPHN PA cultured in media with serum, and after chronic exposure to U46619. b In serum-deprived tissues, decreased DNA synthesis [i] and active protein synthesis [ii] especially following U46619 exposure. Protein content was lower in homogenates of PPHN arteries in media and in U46619 (N = 5; *p < 0.05 compared to control D3 in same treatment group; #p < 0.05 compared to PA from same animal model in media alone)

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