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Fig. 7 | Fibrogenesis & Tissue Repair

Fig. 7

From: Cytoglobin expression in the hepatic stellate cell line HSC-T6 is regulated by extracellular matrix proteins dependent on FAK-signalling

Fig. 7

Analysis of FAK Y397 phosphorylation in HSC-T6 cells cultured different ECM surfaces for 48 h. a Western blot for protein expression of phosphorylated FAK Y397 in cells cultured on laminin, non-coated tissue culture plastic and collagen I with β-Actin loading control. b Mean FAK Y397 FITC fluorescence detected by flow cytometry in HSC-T6 cells cultured on non-coated plastic and collagen I; * denotes significant difference (P < 0.01). The results represent the mean of three experiments ±SD. c, d Confocal image of cells cultured on glass and collagen I and probed for expression of phosphorylated FAK Y397 (green). Nuclei are shown in blue (Hoechst 33342)

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