Fig. 4
a Cygb and αSMA expression in HSC-T6 cells cultured on different ECM proteins analysed by RT-qPCR calibrated to expression on non-coated Flasks. *Denotes significant difference from non-coated control at P < 0.05 according to one-way ANOVA with Tukey’s post hoc test; + denotes significant difference from non-coated control at P < 0.05 according to t test assuming equal variances. The results represent the mean of three experiments ± SD. b Statistically significant (R 2 = 0.913, P < 0.05) negative correlation between αSMA and Cygb expression. c Western blot analysis of Cygb protein expression on non-coated plastic, collagen I and laminin