Fig. 3
a Images of HSC-T6 cells cultured on mixed matrix surfaces of collagen I and laminin in differing concentrations 48 h post seeding at 100,000 cell/ml at 400× magnification. a 10:0 μg/cm2, b 10:5 μg/cm2, c 10:10 μg/cm2, d 5:10 μg/cm2, e 0:10 μg/cm2 collagen I: laminin and f non-coated control. b Confocal images of HSC-T6 cells seeded at 100,000 cells/ml on glass, collagen I and laminin coverslips 48 h post seeding, blue retinoid autofluorescence activated at 351 nm and detected at 515 nm. a glass, b glass zoom, c collagen I, d collagen I zoom, e laminin, f laminin zoom. Image analysis in ImageJ showed a statistically significant 5-fold increase in the fluorescence of cells cultured on laminin in the presence ATRA compared to untreated controls (P < 0.001) from 5.15 ± 2.7 to 27.8 ± 12 relative fluorescence units respectively