Fig. 2
a Effect of ECM different proteins on the growth of HSC-T6 cells; *Denotes significant difference from non-coated control at P < 0.05 according to one-way ANOVA with Tukey’s post hoc t test. Cells were seeded at 100,000 cells/ml and counted on a haemocytometer 48 h post seeding. Cell density is expressed as cells per T25 flask. b Effect of ECM on cell number per T25 across a passage (48 h). Cells from the same population were seeded at 100,000 cells/ml, 5 ml per flask and grown on non-coated and collagen I coated flasks. Cells were counted on a haemocytometer at four different time points. The results represent the mean of three experiments ± SD. Not significant (one way ANOVA P = 0.34). c Cell number per well over 70 h. Cells from the same population were seeded at 100,000 cells/ml, 1 ml per well in a 24 well plate and grown on non-coated plastic, laminin or collagen I. Cell number was quantified by the Cell-IQ analysis software. Cell number on laminin was significantly different (P < 0.01 one-way ANOVA) from cell number on non-coated plastic or on collagen I between 5–45 h of culture, with the exception of hour 25. Number of cells on collagen I were significantly different from cell number on non-coated plastic between 15 and 40 h. The results represent the mean of three experiments ±SD