Fig. 6

Gene expression changes during in vitro reversion of human primary HSCs inversely correlate with changes observed during in vitro HSC activation. a Venn diagram showing the number of genes differentially regulated during HSC activation ([qHSCs vs aHSCs], p ≤ 0.05 and fold change ≥2) and the number of genes differentially regulated during in vitro reversion to quiescence-like ([aHSCs vs rHSCs], p ≤ 0.05 and fold change ≥2). The intersection of the Venn diagram shows the number of overlapping genes between both comparisons. b Expression heatmap for the genes that are significantly upregulated during HSC activation and significantly downregulated during reversion to quiescence-like. c Profile plot showing the expression profile of ACTG2 expression in qHSCs, aHSCs, and rHSCs as illustration for the 148 genes showing a similar expression profile. d The top five Gene Ontology (GO) terms and KEGG pathways associated with the set of genes represented in (c). e Expression heatmap for the genes that are significantly downregulated during HSC activation and significantly upregulated during reversion to quiescent-like cells. f Profile plot showing the expression profile of NFKBIA expression in qHSCs, aHSCs, and rHSCs as illustration for the 64 genes showing a similar expression profile. g The top five GO terms and KEGG pathways associated with the set of genes represented in (f)