Fig. 1From: In vitro reversion of activated primary human hepatic stellate cellsIsolation and culture activation of human primary HSCs. a Quiescent HSC-enriched cell layer (arrow) following an 8 % Nycodenz density gradient centrifugation. b Light microscopic and α-SMA immunocytochemistry images of the freshly isolated, qHSC-enriched cell population and fully culture activated HSCs (passage 4). c Log2 mRNA expression levels of COL1A1 and LOX in freshly isolated, non-plated qHSCs and aHSCs from five different donors. In the graphs, **p < 0.01. d PDGFRβ and GAPDH protein levels in aHSCs from four donors. e Positive immunostainings for the neural markers NCAM1, nestin, and desminBack to article page