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Fig. 1 | Fibrogenesis & Tissue Repair

Fig. 1

From: In vitro reversion of activated primary human hepatic stellate cells

Fig. 1

Isolation and culture activation of human primary HSCs. a Quiescent HSC-enriched cell layer (arrow) following an 8 % Nycodenz density gradient centrifugation. b Light microscopic and α-SMA immunocytochemistry images of the freshly isolated, qHSC-enriched cell population and fully culture activated HSCs (passage 4). c Log2 mRNA expression levels of COL1A1 and LOX in freshly isolated, non-plated qHSCs and aHSCs from five different donors. In the graphs, **p < 0.01. d PDGFRβ and GAPDH protein levels in aHSCs from four donors. e Positive immunostainings for the neural markers NCAM1, nestin, and desmin

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