Fig. 3From: Novel approach for the detection of tubular cell migration into the interstitium during renal fibrosis in ratsMigration of BrdU-positive tubular cells into the interstitium of the UUO kidneys. a Masson’s trichrome staining (a–d) and EVG staining (e–f) of kidney sections. (a, e) Normal kidney; (b, f) contralateral kidney, 10 days; (c, g) UUO kidney, 6 days; and (d, h) UUO kidney, 10 days. Magnification, ×200. b Localization of BrdU-positive cells in the normal (a), contralateral (b), and UUO (c–f) kidneys of rats with long-term BrdU labeling. Magnification, ×1000. Arrowheads indicate BrdU-positive cells protruding from TBM or migrating into interstitium. c Destruction of tubular basement membrane in the UUO kidneys of rats with long-term BrdU labeling. BrdU (red), markers (green), and DAPI (blue). Magnification, ×1000Back to article page