Fig. 7

Overexpression of CCR1, CCR2, and CCR3 in fibrotic mouse skin is reversed by CSD. a Skin tissue sections from mice treated systemically with bleomycin or vehicle and injected daily i.p. with CSD or vehicle were double-stained for CCR1, CCR2, or CCR3 (red) and for HSP47 (green). Nuclei were counterstained using DAPI (blue). Note the enhanced expression of all of these proteins in the skin tissue of bleomycin-treated mice and that CSD treatment almost completely blocked the accumulation of these proteins. Similar results were obtained in three independent mice in each category. b–d Quantification of staining. Representative fields were photographed at 400× magnification. The number of double-positive cells (b, CCR1+/HSP47+; c, CCR2+/HSP47+; d, CCR3+/HSP47+) was counted in three subjects per category, five high power fields (HPF) per subject. The data are presented in terms of the number of double-positive cells per HPF (average ± SEM). ***p < 0.002; **p < 0.01; *p < 0.05