Fig. 6

Overexpression of CCR1, CCR2, and CCR3 and HSP47 in scleroderma lung tissue. Control and SSc lung sections were double-stained for HSP47 (green) and for a CCR1, c CCR2, or e CCR3 (red). Nuclei were counterstained using DAPI (blue). The indicated fields in the third column are shown at high magnification in the fourth column. Note the enhanced expression of all of these proteins in SSc lung compared to Control lung. Note (see arrows) that the green and red staining in double-labeled cells need not overlap. Similar results were obtained in three independent samples in each category. b, d, f Quantification of staining. Representative fields were photographed at 400× magnification. The number of double-positive cells (b, CCR1+/HSP47+; d, CCR2+/HSP47+; f, CCR3+/HSP47+) was counted in three subjects per category, five high power fields (HPF) per subject. The data are presented in terms of the number of double-positive cells per HPF (average ± SEM). ***p < 0.002; **p < 0.01; *p < 0.05