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Fig. 4 | Fibrogenesis & Tissue Repair

Fig. 4

From: Enhanced chemokine-receptor expression, function, and signaling in healthy African American and scleroderma-patient monocytes are regulated by caveolin-1

Fig. 4

Overexpression of CCR1, CCR2, CCR3, and CD68 in scleroderma skin tissue. Control and SSc skin sections were double-stained for the monocyte/macrophage marker CD68 (green) and for a CCR1, c CCR2, or e CCR3 (red). Nuclei were counterstained using DAPI (blue). The indicated fields in the third column are shown at high magnification in the fourth column. Note the enhanced expression of all of these proteins in SSc skin compared to Control skin. Note (see arrows) that the green and red staining in double-labeled cells need not overlap. Similar results were obtained in three independent samples in each category. b, d, f Quantification of staining. Representative fields were photographed at 400× magnification. The number of double-positive cells (b, CCR1+/HSP47+; d, CCR2+/HSP47+; f, CCR3+/HSP47+) was counted in three subjects per category, five high power fields (HPF) per subject. The data are presented in terms of the number of double-positive cells per HPF (average ± SEM). ***p < 0.002; **p < 0.01; *p < 0.05

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