Renal expression of Wnt ligand genes upon unilateral ureter obstruction (A-K). We isolated total RNA from whole kidney tissues 3 and 7 days after ureter ligation and analyzed expression of putative Wnt ligand genes by quantitative real-time PCR. The bar graphs display relative mRNA expression of indicated Wnt ligand genes upon ureter ligation in relation to kidneys of sham-operated control mice. The arrows highlight robust expression of β-catenin in dilated tubules. Data is presented as means ± SD. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001, n.s. no significance, UUO, unilateral ureteral obstruction; P values were calculated respective to control.