Figure 4
Effect of inflammatory, growth factor and lipid mediator (IGL) treatment on collagen levels. Liver slices of 150-μm thickness were cultured either with CCL4 or IGL cocktail for 24 hours, and the expression levels of collagen was quantitated by real-time quantitative PCR using the beta actin gene as an endogenous control (A). Expression levels of each of the genes in the control samples (liver slices cultured with culture media alone, without CCL4 or IGL cocktail) were normalized to 1. Total collagen in the liver slices was estimated (B) using Sirius red dye. Values represent mean ± SEM (n = 4 per group). An unpaired student t-test was used for statistical comparison. **P <0.01 and ***P <0.001, when compared to control.