Figure 2
Steatosis, inflammation and oxidative stress in the liver slices cultured in the inflammatory, growth factor and lipid mediator (IGL) cocktail. Liver slices of 150-μm thickness were cultured either with CCL4 or IGL cocktail for 24 hours, after which triglyceride levels (A) and oxidative stress levels (F) were estimated. RNA was isolated from the liver slices, and expression levels of CRP (B), IL-6 (C), TNF-α (D) and MCP-1 (E) were quantified by real-time quantitative PCR using the beta actin gene as the endogenous control. Expression levels of each of the genes in the control samples (liver slices cultured with culture media alone without CCL4 or IGL cocktail) were normalized to 1. Values represent mean ± SEM (n = 4 per group). An unpaired student t-test was used for statistical comparison. *P <0.05, **P <0.01, and ***P <0.001, when compared to control.