Figure 1

Viability of liver slices. Liver slices of 150 μm thickness were obtained and incubated with Williams E media supplemented with 15% fetal bovine serum and 1% GlutaMAX for indicated time periods. ATP levels were estimated at the end of the experiment, and levels were normalized to total protein content. The ATP levels in the control slices (incubated for 4 hours) were normalized to 100 and the percent ATP levels were calculated accordingly. **P <0.01 and ***P <0.001, when compared to respective samples at 4 hours.