Figure 1

Activation of HSC induced expression of C3aR and C5aR, but not C5L2. Primary HSC were isolated from C57BL/6 mice and grown in culture. Expression of (A) α-SMA and (B) collagen1A1 and (C) C3aR, C5aR, and C5L2 mRNA was measured by qRT-PCR and normalized to 18S. Values represent means ± SEM, N = 5. (A/B) Values with different superscripts are significantly different from each other (P <0.05). (C) *P <0.05 compared to day 2. (D) Confocal immunocytochemistry for C5aR protein expression was assessed in HSC on day 5 and 10 of culture. C5aR (green) and DAPI (blue). Images are representative of three independent HSC isolations.