Figure 2

Antibody administration and cellular infiltration. Animals exposed to AngII for 3 days were concurrently administered mIgG by tail vein injection. The amount of cellular infiltrate within whole cross-sections was assessed using a grid-scoring method of whole myocardial cross-sections after H&E stain and the results reported as percentage of area affected (A). Representative H&E stained sections of AngII (B) and AngII + mIgG (C) are shown at 5x and areas of infiltration are highlighted by black borders. Immunofluorescent staining of CD45 (green) and SMA (red) indicate dual staining (yellow) within areas of infiltration in AngII (D), AngII + mIgG (E) treated myocardium, with representative images shown at 63x and an additional 4x digital magnification of an area of dual staining shown (F). *P <0.05, **P <0.01 compared to saline, ⁺P <0.05, ⁺⁺P <0.01 compared to AngII.