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Figure 5 | Fibrogenesis & Tissue Repair

Figure 5

From: Impaired Cav-1 expression in SSc mesenchymal cells upregulates VEGF signaling: a link between vascular involvement and fibrosis

Figure 5

Downregulation of Cav-1 in HC-MSCs by Cav-1-siRNA impaired VEGF signaling. (A) HC-MSC was transfected with specific Cav-1-siRNA (siRNA) or non-targeting siRNA (NT), and Cav-1 protein expression was evaluated by western blot. The cells transfected with Cav-1-siRNA showed a decreased protein expression of Cav-1 when compared with cells transfected with NT siRNA. (B) Proteins immunoprecipitated (IP) by VEGFR2 antibodies were fractionated by SDS-polyacrylamide gel electrophoresis. The immunoblots were probed, by using specific antibodies against phosphotyrosine (pY) and p85. In siRNA HC-MSCs, after VEGF treatment, the tyrosine phosphorylation of VEGFR2 was significantly increased when compared to NT MSCs. (C) Western blot of CTGF expression. VEGF stimulation significantly increased CTGF proteins levels in siRNA HC-MSCs, when compared to NT HC-MSCs. Each experimental condition was performed in triplicate. Blot was representative of all the experiments. The values were expressed as arbitrary unit of optical density (OD) (*P <0.01; **P <0.001; ***P <0.0001).

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