Tranilast effects on fibrosis in the diaphragm and TA of mdx mice. At endpoint, the diaphragm muscle of vehicle-treated C57BL/10 (A), vehicle-treated mdx (B), tranilast-treated C57BL/10 (C) or tranilast-treated mdx (D) mice was excised and collagen infiltration visualised in cross-sections after Van Gieson’s staining. (E) Fibrotic accumulation in Van Gieson’s stained sections from diaphragm and tibialis anterior muscles of untreated and treated mice. #P < 0.05 group main effect, mdx vs. control. *P < 0.05 mdx treated vs. mdx control; n.s, not significant.