Table 1 Technical evaluation of the BGM competitive ELISA
Technical validation step | BGM ELISA |
|---|---|
Target | MMP-9 and -12 degradation of biglycan |
Lower limit of detection (LDL) | 1.54 ng/mL |
Dilution recovery of human serum | 100% (1:2), 103% (1:4) |
Dilution recovery of human plasma | 97% (1:2), 99% (1:4) |
Dilution recovery of rat serum | 107% (1:2), 102% (1:4) |
Dilution recovery of rat plasma | 89% (1:2) |
Dilution recovery of mouse serum | 105% (1:2) |
Dilution recovery of mouse plasma | 97% (1:2) |
Intra-assay variation | 10% |
Inter-assay variation | 15% |