Figure 5

Cyclosporin A delays dermal fibroblasts wound migration in vitro . Wounded, monolayered dermal fibroblasts from nude (A) and wild type (B) mice were cultured for 36 hours with CsA at concentrations of 100 ng/ml or 1000 ng/ml. Migration was expressed as a percentage of distance between post-wounded edges of monolayered dermal fibroblasts. Triplicate wells were used and the experiment was repeated three times (n = 9). Error bars represent SEM; ***p value <0.001 calculated from the differences between control and CsA treated cultures at 6, 12 and 24 h.