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Figure 3 | Fibrogenesis & Tissue Repair

Figure 3

From: Cyclosporin A reduces matrix metalloproteinases and collagen expression in dermal fibroblasts from regenerative FOXN1 deficient (nude) mice

Figure 3

Cyclosporin A reduces protein kinase C (PKC) signaling in nude dermal fibroblasts. Primary dermal fibroblast from nude and wild type mice were treated with varying doses of CsA (0.1-1000 ng/ml) for 24 h. Cell lysates was harvested and 40 microgram of total protein per sample was separated on 12% electrophoresis gel. The levels of α, β, ε PKC and phosphorylated-PKC (pan) were determined by immunobloting with specific antibodies. All samples were immunoblotted for ß-actin to assess variation in protein loading. The levels of α, β, ε PKCs (A) and phosphorylated-PKC (B) are shown.

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