Immunophenotypic characterization of freshly isolated dermal fibroblasts from nude and B6 mice by flow cytometric analysis. Cells were labeled with phycoerythrine-conjugated antibodies against Sca-1, CD117, Oct3/4, CD90, CD73 and CD44. (A) The data (% of labeled cells) represent the mean of three experiments. Each experiment consisted of a pool of cells collected from three animals. Asterisks indicate significant differences between nude and B6 dermal fibroblasts (** p < 0.01; ***p < 0.001). (B) Representative flow cytometric analysis of dermal fibroblasts. The percentage of cells staining positive is indicated on each panel.