Molecular constituents of the extracellular matrix in rat liver mounting a hepatic progenitor cell response for tissue repair

Table 3 Applied antibodies for immunohistochemistry and immunofluorescence

Primary antibody	Dilution	Catalog number (clone)	Secondary antibody	Dilution	Catalog number
Immunohistochemistry
^1,*Matriptase	1:500	AF3946	⁷Vectastain Elite ABC	Not applicable	PK-6106
^1,*HAI-1	1:500	AF1141	⁷Vectastain Elite ABC	Not applicable	PK-6105
^2,+Nidogen-1	1:1000	ab14511	⁴Dako EnVision + System-HRP	Not applicable	K4008
³DLK1	1:50	N/A	⁴Dako EnVision + System-HRP	Not applicable	K4008
Immunofluorescence
¹Integrin-β6	1:50	AF2389	⁸Alexa Fluor 488/594	1:400	A-11055/A-11058
¹Agrin	1:100	AF550	⁸Alexa Fluor 488/594	1:400	A-11055/A-11058
¹h/rOV-6	1:50	MAB2020 (Clone OV-6)	⁹Fluorescein	1:200	715-095-151
¹HAI-1	1:50	AF1141	⁸Alexa Fluor 488/594	1:400	A-11055/A-11058
¹DLK1	1:100	MAB114 (211309)	⁸Alexa Fluor 488	1:400	A-21141
³DLK1	1:250	N/A	⁸Alexa Fluor 594	1:400	A-21207
⁴Desmin	1:500	M0760 (D33)	⁹Fluorescein	1:200	715-096-151
⁴Laminin	1:2000	Z0097	⁸Alexa Fluor 594	1:400	A-11037/A-21207
⁵COL1A1	1:100	AB755p	⁸Alexa Fluor 594	1:400	A-11037/A-21207
²Nidogen-1	1:1500	ab14511	⁸Alexa Fluor 594	1:400	A-11037/A-21207
⁶Ki67	1:2000	NCL-Ki67p	⁸Alexa Fluor 594	1:400	A-11037/A-21207

¹R&D Systems, Inc. Minneapolis, MN USA; ²AbCAM, Cambridge, UK; ³Polyclonal rabbit antibody; ⁴Dako A/S, Glostrup, Denmark; ⁵Milipore, Billerica, MA USA; ⁶Leica Microsystems Inc., Buffalo Grove, IL USA; ⁷Vector laboratories, Burlingame, CA USA; ⁸Life Technologies Europe BV, Nærum, Denmark; ⁹Jackson ImmunoResearch Europe Ltd., Suffolk, UK; *Sections were boiled in retrieval solution (St14) or retrieval solution, pH9 (Spint1) (Dako, catalog number S2367/S1699) for 20 min in a microwave oven and let to cool to room temperature for 20 min. ⁺Sections were digested in proteinase K for 2.5 minutes at room temperature (Dako, catalog number S3020).

ISSN: 1755-1536