Table 2 Applied primers and probes for real-time RT-PCR
Gene symbol | Sequence | Threshold/concentration |
|---|---|---|
Krt19 | 0.052 | |
1Primer forward | 5′-GGC GCC ACC ATT GAG AACT-3′ | 100 nM |
1Primer reverse | 5′-GCC AGG CGG GCA TTG-3′ | 100 nM |
1Probe | 5′-FAM-CAA GAT AGT CCT ACA GAT CG-BHQ-1-3′ | 200 nM |
Spint1 | 0.1 | |
1Primer forward | 5′-GCA TCT ACT GCA AGG TGA CCA A-3′ | 300 nM |
1Primer reverse | 5′-CCA CAG CTC AAC TTC TTC TGG AT-3′ | 300 nM |
2Probe | 5′-FAM-TGT CAG TGT TAA GAG AAA C-MGBNFQ-3′ | 280 nM |
Spint2 | 0.1 | |
1Primer forward | 5′-TGC TCT GGG AAG CAG ATG TAT C-3′ | 600 nM |
1Primer reverse | 5′-TGC GAA CCA TTC GGA TCA G-3′ | 600 nM |
2Probe | 5′-FAM-ACA AAA GCG GTG ATC C-MGBNFQ-3′ | 100 nM |
St14 | 0.1 | |
1Primer forward | 5′-TGG CTG TGG AGC GAG TTG T-3′ | 700 nM |
1Primer reverse | 5′-TGC CAC GGG CAT GGA-3′ | 700 nM |
1Probe | 5′-FAM-CCT TCC CCA TTG ACC CCA GAA TGC-BHQ-1-3′ | 240 nM |
Prss8 | 0.1 | |
1Primer forward | 5′-CAG TCA AGA ATC GGA GCT GAT G-3′ | 300 nM |
1Primer reverse | 5′-CAC TGC CAC CAC CTG TGA TG-3′ | 300 nM |
1Probe | 5′-FAM-CTG AAG CTT CCT GTG GTG CAG TCA TCC-BHQ-1-3′ | 140 nM |