Table 1 Identification of ECM constituents modulated in first and second tiers of defense upon hepatic injury
Protein name | Gene symbol | Modulation of transcriptional profile | Product–moment correlation, coefficient r | |
|---|---|---|---|---|
First tier of defense using the PHx protocol as model system | Second tier of defense using the AAF/PHx protocol as model system | Krt1-19-geneX in second tier of defense using the AAF/PHx protocol as model system | ||
Hepatic progenitor cell markersa | ||||
Keratin 19 | Krt1-19 | ⇅ | ↑ * | +++++ M |
α-Fetoprotein | Afp | ⇅ | ↑ * | +++++ M |
Epithelial cell adhesion molecule, EpCam | Tacstd1 | ⇅ | ↑ * | +++++ M |
Delta-like homolog 1 | Dlk1 | ⇅ | ↑ * | +++++ M |
Deleted in malignant brain tumor 1 | Dmbt1 | ⇅ | ↑ * | +++++ M |
Glutathione S-transferase, pi | Gstp1 | ⇅ | ↑ * | +++++ M |
Gap junction protein, α1 (Cx43) | Gja1 | ⇅ | ↑ * | +++++ M |
Prominin, CD133 | Prom1 | ⇅ | ↑ * | +++++ M |
CD24 | Cd 24 | ⇅ | ↑ * | +++++ M |
Vimentin | Vim | ⇅ | ↑ * | +++++ M |
Proliferating cell nuclear antigen | PCNA | ↑ * | ↑ | +++++ |
Mesenchymal cell markers | ||||
Desmin | Des | ↑ | ↑ | +++ |
S100 calcium-binding protein A4 | S100a4 | ↑ | ↑ | ++++ |
Secreted phosphoprotein 1 (osteopontin) | Spp1 | ↑ | ↑ | ++++ |
Extracellular matrix constituents | ||||
Basal lamina and ECM structural constituents | ||||
Agrin | Agrn | ↓ * | ↑ * | +++++ M |
Biglycan | Bgn | ↓ * | ↑ * | +++++ M |
Decorin | Dcn | ↑ * | ↑ | ++++ |
Elastin | Eln | ↑ * | ↑ | +++++ |
Fibrillin 1 | Fbn1 | ⇅ | ↑ * | +++++ M |
Fibronectin 1 | Fn1 | ↓ * | ↑ * | +++ |
Fibulin 1 | Fbln1 | ↓ | ↓ | ++++ |
Collagens | Col1a1 | ↑ | ↑ | +++++ |
Col1a2 | ↑ | ↑ * | +++++ | |
Col3a1 | ↑ * | ↑ * | +++++ | |
Col4a1 | ⇅ | ↑ * | +++++ M | |
Col5a1 | ↑ | ↑ * | +++++ | |
Col5a2 | ↑ | ↑ * | +++++ | |
Col6a1 | ↑ | ↑ * | +++++ | |
Col6a3 | ↑ | ↑ * | +++++ | |
Col8a1 | ↓ | ↑ * | +++++ | |
Col9a3 | ⇅ | ↑ * | +++++ M | |
Col14a1 | ↑ * | ↑ * | ++++ | |
Col16a1 | ↑ | ↑ * | +++++ | |
Col18a1 | ↓ | ⇅ | +++ | |
Laminins | Lama2 | ↓ * | ↑ | +++++ |
Lama5 | ⇅ | ↑ * | ++ M | |
Lamb1 | ⇅ | ↑ * | +++++ M | |
Lamb2 | ↓ * | ↑ * | ++++ M | |
Lamc1 | ↑ * | ↑ * | ++ | |
Lamc2 | ⇅ | ↑ | +++++ | |
Integrins | Itga1 | ↑ | ↓ | ++ |
Itga4 | ↓ | ↑ * | ++++ M | |
Itga5 | ↓ | ↓ | +++ | |
Itga7 | ↓ | ↓ * | +++ | |
Itgb1 | ↑ | ↑ * | ++++ | |
Itgb2 | ↓ * | ↑ * | +++++ M | |
Itgb4 | ⇅ | ↑ * | +++++ M | |
Itgb6 | ⇅ | ↑ * | +++++ M | |
Itgb7 | ↑ | ↑ * | +++ | |
Nidogens | Nid1 | ↑ * | ↑ * | +++++ M |
Nid2 | ↓ | ↑ * | +++++ M | |
Secreted protein, acidic, cysteine-rich, (osteonectin, SPARC) | Sparc | ↑ * | ↑ * | +++++ |
ECM proteases and inhibitors | ||||
Matrix metallopeptidases | Mmp2 | ↓ | ↓ | ++++ |
Mmp9 | ⇅ | ⇅ | +++ | |
Mmp12 | ↑ | ↑ * | +++++ M | |
Mmp14 | ↓ | ↑ * | +++ | |
Mmp16 | ↑ | ↑ | +++ | |
Mmp19 | ⇅ | ↑ * | +++ | |
Mmp23 | ↑ | ↑ * | ++++ M | |
TIMP metallopeptidase inhibitor 1 | Timp1 | ↑ | ↑ * | ++ |
A disintegrin and metalloproteinase with thrombospondin motifs 1 | Adamts1 | ↓ | ↑ | +++++ |
Suppression of tumorigenicity 14 (matriptase) | St14 | ⇅ | ↑ * | +++++ M |
Other ECM associated molecules | ||||
Connective tissue growth factor | Ctgf | ⇅ | ↑ * | +++++ M |
Elastin microfibril interfacer 1 | Emilin1 | ↑ | ↑ * | ++++ |
Glypican 3 | Gpc3 | ↓ | ↑ * | +++++ M |
Periostin, osteoblast specific factor | Postn | ↑ | ↑ | +++++ |
Syndecans | Sdc1 | ↑ * | ↑ | ++++ |
Sdc2 | ↓ * | ↓ * | +++ | |
Matriptase (St14) network components | ||||
Hepatocyte growth factor | Hgf | ⇅ | ↑ * | +++ |
Hepatocyte growth factor activator | Hgfac | ↓ | ↓ | +++ |
Hepsin | Hpn | ↓ | ↓ * | +++ |
Kallikrein B, plasma 1 | Klkb1 | ↓ | ↓ * | ++++ |
Plasminogen | Plg | ↓ * | ↓ * | +++ |
Plasminogen activator, urokinase | Plau | ↑ | ↑ * | +++ |
Protease, serine, 1 (trypsin 1) | Prss1 | ⇅ | ↑ | +++++ |
Protease, serine, 8 (prostasin) | Prss8 | ↑ | ↑ | ++++ |
Serine peptidase inhibitor, Kunitz type 1 (HAI-1) | Spint1 | ⇅ | ↑ | +++++ |
Serine peptidase inhibitor, Kunitz type 2 (HAI-2) | Spint2 | ↓ * | ↓ * | ++ |
Serine peptidase inhibitor, clade A (α-1 antiproteinase), member 1 | Serpina1 | ↓ * | ↓ | +++ |
Serine peptidase inhibitor, clade C (antithrombin), member 1 | Serpinc1 | ↓ | ↓ | +++++ |
Serine peptidase inhibitor, clade F (α-2 antiplasmin), member 1 | Serpinf2 | ↓ * | ↓ * | +++++ |