Figure 8

Serum amyloid P (SAP) has no significant effect on the production of hydrogen peroxide induced by N- formyl- methionine- leucine- phenylalanine (fMLP), tumor necrosis factor (TNF) α, phorbol 12-myristate 13-acetate (PMA), or phorbol 12, 13-dibutyrate (PDBu). (A) Granulocytes pretreated with or without SAP were treated with fMLP in the presence of scopoletin. The fluorescence intensity of scopoletin was measured over 3 h, and after measuring the effect of different amounts of hydrogen peroxide on scopoletin fluorescence; this was then converted to hydrogen peroxide production. Similar assays were performed using granulocytes stimulated with (B) TNFα, (C) PDBu, or (D) PMA. All of the figures represent one of the three separate experiments for TNFα, fMLP, or PMA, or one of the two separate experiments for PDBu.