Figure 5

Serum amyloid P (SAP) has no significant effect on the number of some surface receptors associated with granulocyte activation. (A) Human granulocytes were treated with interleukin (IL)-8, granulocyte macrophage colony-stimulating factor (GM-CSF), or tumor necrosis factor (TNF)α in the presence or absence of SAP. The cells were then stained for CD11b. Values are mean ± SEM, n = 3. (B) Granulocytes were treated with GM-CSF or TNFα in the presence or absence of SAP. The cells were then stained for CD62L. Values are mean ± SEM, n = 3. (C) Granulocytes were treated with IL-8 or TNFα in the presence or absence of SAP. The cells were then stained for CD32. (D) Granulocytes were treated as in (C), and were then stained with mouse IgG1. The absence of an error bar indicates that the error was smaller than the line thickness.