Figure 4

Serum amyloid P (SAP) inhibits murine granulocyte adhesion induced by tumor necrosis factor (TNF)α. First, 96-well tissue culture plates were coated with plasma fibronectin. Crude murine granulocytes were incubated with SAP and/or TNFα in the plates, and adhered cells were stained for the granulocyte marker Ly6G and counted. Values are mean ± SEM, n = 8. *P <0.05, ***P <0.001 (one-way analysis of variance (ANOVA), Tukey’s test).