Figure 11

Serum amyloid P (SAP) decreases the accumulation of Ly6G-positive cells in 0. 2 U/ kg bleomycin- treated mouse lungs. Mice were treated with 0.2 U/kg bleomycin using an oropharyngeal technique on day 0. Mice were then injected with either 50 μg SAP or an equal volume of buffer on days 1 and 2. After the mice were killed on day 3, cells were collected by bronchoalveolar lavage (BAL). (A) The total number of cells collected by BAL from mice with the indicated treatments. (B) Cells collected by BAL were stained with anti-mouse Ly6G to detect granulocytes. Arrows indicate Ly6G-positive cells. Bars are 20 μm. (C) The total number of Ly6G-positive cells in the above experimental groups. Values are mean ± SEM (n = 4 for control and mice treated with bleomycin and buffer, n = 5 for mice treated with saline and buffer or mice treated with bleomycin and mouse SAP, and n = 6 for mice treated with bleomycin and human SAP). Using the non-parametric Mann–Whitney two-tailed t test, there was an increased number of Ly6G-positive cells in the BAL from bleomycin and buffer when compared to control, mice treated with saline and buffer, or mice treated with bleomycin and human SAP; *P <0.05. Using the non-parametric Mann–Whitney two-tailed t test, there was no significant difference between mice treated with bleomycin and buffer or mice treated with bleomycin and mouse SAP. However, using the non-parametric Mann–Whitney one-tailed t test, there was a statistically significant increase in the number of Ly6G-positive cells in the BAL from mice treated with bleomycin and buffer when compared to the number of Ly6G-positive cells in the BAL from mice treated with bleomycin and mouse SAP. (D) After obtaining cells by BAL, lung sections were stained with anti-mouse Ly6G to detect granulocytes. Arrows indicate Ly6G-positive cells. Bars are 100 μm. (E) Counts of granulocytes in sections. Values are mean ± SEM, n = 3. *P <0.05 (t test).