Figure 1

Characterization of isolated granulocytes. (A) Flow cytometry showing forward-scatter (x-axis) and side-scatter characteristics (y-axis) from 10,000 events. The P2 area shows the granulocyte population. (B) The number of granulocytes isolated exceeds the number of other non-granulocyte cells. The results are mean ± SEM of percentage total cells (n = 3 separate experiments). (C) Human granulocytes were seeded on a glass slide and spread by centrifugation. Cells were stained with 4',6-diamidino-2-phenylindole (DAPI) to visualize nuclei. Bar is 20 μm. (D) The number of granulocytes was counted based on nuclear morphology. There were fewer non-granulocyte cells compared to the isolated granulocytes. The results are mean ± SEM of percentage total cells (n = 3 separate experiments). ***P <0.001 (t test).