Figure 3

Pulmonary expression of miR-21 and miR-34a in bleomycin treated and control C57BL/6J mice. Mice were treated with100 U/kg bleomycin through mini-osmotic pumps and lung tissue harvested six weeks later. In situ hybridization of miR-21 in (A) bleomycin treated lungs and (B) control lungs. In situ hybridization of miR-34a in (C) bleomycin treated lungs and (D) control lungs. (E) In situ hybridization using a scrambled probe as a negative control. No positive cells were identified when using the scrambled probe in control or bleomycin treated lungs. Magnification 400×, insert magnification 1000×. (F) Quantification of 10 random fields per lung for miR-21 and miR-34a positive cells per mm² ± standard deviation of n = 4 to 9 mice per group. * indicates a significance difference between groups, P < 0.005.