Figure 2

Pulmonary microRNA profile of bleomycin treated and control C57BL/6J mice. Mice were treated with 100 U/kg bleomycin through mini-osmotic pumps and lung tissue harvested three or six weeks later. (A) 11 microRNA were identified as being differentially expressed (FDR < 0.01) in lung clustering the treated and control mice separately. Relative expression is log₂ transformed. Yellow indicates over expression, blue indicates under expression compared to a reference expression level. N = 3 mice per group. (B) MicroRNA expression in the lungs of bleomycin treated at six weeks and control mice, relative to the U6 control, was assessed by qRT-PCR. (C) MicroRNA expression in the lungs of bleomycin treated at three weeks and control mice, relative to U6 control, was assessed by qRT-PCR. Average ± standard deviation of n = 5 to 8 mice per group. * indicates a significant difference between groups, P < 0.05.